Effects of prostate cancer cells on osteoblasts

Primary murine osteoblasts were isolated form the calvariae of newborn mice. 10 days after the addition of ascorbic acid (50 g/ml) and -glycerophosphate (10 mM), cells were serum-starved over night and then incubated for 6 hours with condtioned medium of MDA-PCa2b cells or conditioned medium of PC-3 cells

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Schulze J, Albers J, Baranowsky A, Keller J, Spiro A, Streichert T, Zustin J, Amling M, Schinke T