Ath29 congenic mice - gene expression in aorta

A congenic mouse line was constructed by introgressing a C3H chromosome 9 region harboring Ath29 into the C57BL/6 apoE-deficient background. RNA was extracted from aorta using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA.

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Manichaikul A, Wang Q, Shi YL, Zhang Z, Leitinger N, Shi W