Limited macrophage dynamics in progressing or regressing murine atherosclerotic plaques

Previous studies using particle labeled monocytes to assess recruitment into tissues have demonstrated that bead labeling did not affect the ability of monocytes to enter tissues (Tacke et al., 2007), and particle uptake did not activate p38/MAPK or NFB pathways (Yates and Russell, 2005). However, the possibility remains that bead labeling could have effects on global gene expression profiles. To address this prospect, we performed gene expression profiling of classical and non-classical monocytes that had taken up latex particle in vivo against cells from the same mouse that had not taken up particles.

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